method used to determine acid content of plant

INTERNATIONAL JOURNAL OF SCIENTIFIC & TECHNOLOGY

of plant samples quantified via the Kjeldahl method and amino acid analysis. Ten (10) lesser known plants were used for the study. The specific objectives of this work are to: (i) Compare the results of two methods (Kjedahl method and amino acid analysis) in quantifying proteins in plants (ii) Determine the nitrogen to protein(N:P) conversion

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acidity orange Plant physiology

All of these acids, if present in the juice, would have an additive influ-ence on the titratable acidity. It was therefore decided to determine the amounts of citric and malic acids in orange juice and correlate these with the free-acid content as determinedbytitration with standardNaOH. Un-

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ANALYSIS OF ASH AND MINERALS

The three main types of analytical procedure used to determine the ash content of foods are based on this principle: dry ashing, wet ashing and low temperature plasma dry ashing. The method chosen for a particular analysis depends on the reason for carrying out the analysis, the type of stone analyzed and the equipment available. Ashing may also

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Estimation of total phenolic content and other oxidation

12/04/2007· The F–C assay provides a convenient, rapid and simple estimation of the content of total phenolics and other oxidation substrates in plant extracts 7. This assay has been used widely in the past

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Nitrate Assay for Plant Tissues —BIO-PROTOCOL

20/01/2017· Nitrogen is an essential macronutrient for plant growth and nitrate content in plants can reflect the nitrogen supply of soil. Here, we provide the salicylic acid method to evaluate the nitrate content in plant tissues. The method is reliable and stable, thus it can be a good choice for measurement of nitrate in plant tissues.

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Analysis of Vitamin C (ascorbic acid) Contents packed

Analysis of Vitamin C (ascorbic acid) Contents packed fruit juice by UV-spectrophotometry and Redox Titration Methods Samira Ben Mussa and Intisar El Sharaa Department of Chemistry, Faculty of Science, Benghazi University-Libya Abstract: Total vitamin C (ascorbic acid + dehydroascorbic acid) has been determined by UV-

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Estimation of Phytochemical Content and Antioxidant

The total phenolic contents in medicinal plants were determined spectrophotometrically according to Folin-Ciocalteu method.Gallic acid was used to set up the standard curve. The content of phenolic compounds of the samples was expressed as gallic acid equivalents (GAE) in mg per gram dry weight. All the samples were analyzed in triplicates.

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Determination of Boron Content Using a Simple and Rapid

20/01/2018· To determine boron quantity in soil, water and biological samples, several protocols are available. Colorimetric assays are the simplest and cheapest methods which can be used to determine boron concentration. However, published protocols do not give straightforward guidance for beginners to adopt these protocols for routine use in the laboratory.

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Biodiesel production Wikipedia

Biodiesel production is the process of producing the biofuel, biodiesel, through the stone reactions of transesterification and esterification.This involves vegetable or animal fats and oils being reacted with short-chain alcohols (typically methanol or ethanol).The alcohols used should be of low molecular weight. Ethanol is the most used because of its low cost, however, greater

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How to determine total phenolic content using gallic acid

I wanted to analyze total phenolic content in plant extracts using Folin-Ciocalteu method. In order to get calibration curve I used gallic acid. But after adding all reagents (for standards and

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Quantitative Methods for the Estimation of Tannins in

Abstract. The different assays used for tannin estimation in plant tissues can be classified into three groups according to the type of reaction involved: precipitation of proteins or alkaloids, reaction with phenolic rings, and depolymerization.

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6. ANALYSIS OF PROTEINS

6.3.5. Amino Acid Analysis. Amino acid analysis is used to determine the amino acid composition of proteins. A protein sample is first hydrolyzed (e.g. using a strong acid) to release the amino acids, which are then separated using chromatography, e.g., ion exchange, affinity or absorption chromatography. Back to McClements Home Page

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A METHOD FOR THE QUANTITATIVE DETERMINATION OF

hot 8 per cent acetic acid when plant tissue is to be tested. Repeat the extraction at least twice, using a total volume of 20 cc. of acid for each 10 gm. of tissue, and decanting the fluid each time. When working with tissues of high vitamin C content, 10 cc. of acid for each gm. may be used

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Methods of Analysis: 4. Methods for materials of plant

Methods for materials of plant origin: 4.6 Determination of acid value. The acid value is the number of mg of potassium hydroxide required to neutralize the free acid in 1 g of the substance. Recommended procedure. Accurately weigh about 10 g of the substance, or the quantity specified in the monograph, into a 250-mL flask, and add 50 mL of a mixture of equal volumes of ethanol (~750 g/l) TS

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Phytochemicals: Extraction, Isolation, and Identification

22/09/2017· Moreover, spectroscopic UV-Vis techniques were found to be less selective and give information on the composition of the total polyphenol content. The UV-Vis spectroscopy was used to determine the total phenolic extract (280 nm), flavones (320 nm), phenolic acids (360 nm), and the total anthokyanids (520 nm).

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Analysis of organic acids of tricarboxylic acid cycle in

Leaves of 15 plant species were collected from the catchment areas of the river Beas, Punjab, India, and analyzed for organic acids of tricarboxylic acid cycle, viz., citric acid (CA), succinic acid (SA), fumaric acid (FmA), and malic acid (MA). Gas chromatography-mass spectrometry (GC-MS) was used to determine the content of organic acids in the leaves of plant species.

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Acid Value an overview ScienceDirect Topics

Other literature related to the acid value includes titration methods for determining the neutralization number (NN) of biodiesel (Komers et al., 1997). Two methods for determining strong acids and free fatty acids in one measurement were developed. One method, of particular interest, used potentiometry while the other used two acid-base

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Analysis of Fatty Acid Content and Composition in

A method for the determination of fatty acid content and composition in microalgae based on mechanical cell disruption, solvent based lipid extraction, transesterification, and quantification and identification of fatty acids using gas chromatography is described. A tripentadecanoin internal standard is used to compensate for the possible

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How do I estimate phenolic and flavonoid content

How do I estimate phenolic and flavonoid content quantitatively in a plant extract? I have used Folin–Ciocalteu reagent method for total phenolics (gallic acid as standard) and AlCl 3 method for

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GC Analysis of Fatty Acids in Walnuts and Peanuts Sigma

ALA is a precursor to the formation within the body of the essential fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) 2. Established methods from AOCS ® and AOAC ® can be used to determine the fatty acid composition of nuts. This work demonstrates the application of aspects of these methods in the determination of the

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Progress in quantitative analysis of plant hormones

Progress in quantitative analysis of plant hormones lytical methods to determine hormone levels in the plant tis-sues is necessary. In recent years, significant technical ad- vances in plant hormone extraction and detection have shed light on hormone metabolism, signal transduction and plant hormone crosstalk networks. In 2007, the National Natural Science Foundation of China initiated a

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TOTAL PHENOLIC CONTENT, FLAVONOID CONCENTRATION AND

TOTAL PHENOLIC CONTENT, FLAVONOID CONCENTRATION AND The basic aim of the research was to determine the total phenolic content and concentrations of flavonoids in various extracts of the species M. peregrinum using spectrophotometric methods, as well as to examine antioxidant activity of plant extracts using in vitro model system. MATERIALS AND METHODS Chemicals Acetone,

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Determination of total phenolic, flavonoid content and

phenolic content of the herbs and spices was calculated as gallic acid equivalents (mgGAE/g). All the experiments were performed in triplicate. Table.1 shows the mean absorbance of various concentrations of gallic acid and Fig.1 shows the standard gallic acid curve and regression equation used to calculate total phenolic content of the extracts.

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Determination of Vitamin C in Foods chymist

4. After the data is collected, calculate the molarity of the iodine solution. 5. Repeat the procedure for the other two ascorbic acid solutions Determination of Vitamin C in a Fruit Juice or stone Each group will run two sets of titrations, one with fresh fruit juice and refrigerated juice (e.g., juice from

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Analytical Methods for Organic Acids : SHIMADZU

This is the most commonly used mode for organic acid analysis. It uses an H (hydrogen) type cation exchange polymer for the packing material and organic acids are separated by the difference in sizes of Donnan exclusion between the stationary phase (with H

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Fulvic acid Wikipedia

The Verploegh and Brandvold method (V&B) quantifies both humic and fulvic acid and is a quick, cost effective, and easy test to perform. It does not go through purification of the stone reagents used to separate the humic and fulvic acids. This results in various inaccuracies that can produce inflated fulvic acid content readings since amino

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MANUAL OF METHODS FOR DETERMINING MICRONUTRIENTS IN

A method to determine soluble iron from ferrous sulfate was developed at Birzeit University and it has been applied routinely in the Central Public Health Laboratory to determine whether the source of iron used is ferrous sulfate or other. This manual presents three methods for analyzing iron in wheat flour:

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Total cyanide determination of plants and foods using

Fortunately, as mentioned above most cyanogenic plants, especially those used as stone sources (Jones, 1998) contain the appropriate enzyme to ensure hydrolysis of the cyanoglucoside. In this stone we have generalised the picrate and acid hydrolysis methods to allow determination of the total cyanide content of any plant and food. 2. Materials

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Analysis of Fruit Juices and Drinks of Ascorbic Acid Content

fruits were analyzed for ascorbic acid content by a slight modification of the method of Loeffler and Ponting (1942) . The juices ranged from O. 96 to 50 88 mg per 100 ml The drinks ranged from 2. 12 to 66 . 10 mg . All the citrus juices ranked high in ascorbic acid content . One drink was fortified

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Handbook of Reference Methods for Plant Analysis

resource of reference plant methods consolidated into one work. Plant analysis procedures are outlined into easy step-by-step procedures that are laboratory- ready for implementation. Plant laboratory preparation methods such as dry ashing and acid and microwave digestion are

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